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rabbit anti cd133 fitc conjugated antibody (Bioss)

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Bioss rabbit anti cd133 fitc conjugated antibody
Rabbit Anti Cd133 Fitc Conjugated Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+cd133+fitc+conjugated+antibody/pm33536065-87-16-21?v=Bioss
Average 90 stars, based on 2 article reviews

rabbit anti cd133 fitc conjugated antibody - by Bioz Stars, 2026-08

90/100 stars

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Phagocytosis and vasculogenesis functions of BM-EPCs of liver fibrosis rats. A: Bone marrow-derived endothelial progenitor cells uptake of Dil-ac-LDL (red); B: BM-EPCs binding with FITC-UEA-1 (green); C: Merge of A and B (yellow); D: Vascular network-like structures of BM-EPCs on Matrigel. Bars: 50 μm (A-C); 200 μm (D); BM-EPCs: Bone marrow-derived endothelial progenitor cells.

Journal: World Journal of Gastroenterology

Article Title: Transplantation of bone marrow-derived endothelial progenitor cells and hepatocyte stem cells from liver fibrosis rats ameliorates liver fibrosis

doi: 10.3748/wjg.v24.i2.237

Figure Lengend Snippet: Phagocytosis and vasculogenesis functions of BM-EPCs of liver fibrosis rats. A: Bone marrow-derived endothelial progenitor cells uptake of Dil-ac-LDL (red); B: BM-EPCs binding with FITC-UEA-1 (green); C: Merge of A and B (yellow); D: Vascular network-like structures of BM-EPCs on Matrigel. Bars: 50 μm (A-C); 200 μm (D); BM-EPCs: Bone marrow-derived endothelial progenitor cells.

Article Snippet: After 10 d of culture under inducing conditions, 2 × 10 6 BM-EPCs were incubated with the FcR blocking reagent (Miltenyi Biotec Inc., Auburn, CA, United States) and fluorescein isothiocyanate (FITC)-conjugated rabbit anti-rat CD133 antibody (BD Biosciences) and rabbit anti-rat vascular endothelial growth factor receptor 2 (VEGFR2) antibody (BD Biosciences) for 30 min at 4 °C, respectively.

Techniques: Derivative Assay, Binding Assay

Evaluation of the expression of CD133 in cervical cancer stem cells. (A) Results of immunofluorescence, the FACS-sorted cervical cancer stem-like side population cells showed increased positivity to the CD133 stem cell surface protein. Magnification, ×400. (B) Quantification of the results of reverse transcription-quantitative polymerase chain reaction and (C) western blot analyses revealed that the relative mRNA and protein expression levels of CD133 were significantly elevated in the SP cells, compared with the MP cells. Equal concentrations of protein were loaded per lane. GAPDH was used as a loading control. (D) Relative expression levels of CD133 compared with GAPDH in SP and MP cells, from the western blot analysis. Data are presented as the mean ± standard error of the mean. ** P<0.01, vs. SP cells. FACS, fluorescence-activated cell sorting; SP, side population; MP, main population; CD, cluster of differentiation.

Journal: Molecular Medicine Reports

Article Title: Production of interleukin-4 in CD133 + cervical cancer stem cells promotes resistance to apoptosis and initiates tumor growth

doi: 10.3892/mmr.2016.5195

Figure Lengend Snippet: Evaluation of the expression of CD133 in cervical cancer stem cells. (A) Results of immunofluorescence, the FACS-sorted cervical cancer stem-like side population cells showed increased positivity to the CD133 stem cell surface protein. Magnification, ×400. (B) Quantification of the results of reverse transcription-quantitative polymerase chain reaction and (C) western blot analyses revealed that the relative mRNA and protein expression levels of CD133 were significantly elevated in the SP cells, compared with the MP cells. Equal concentrations of protein were loaded per lane. GAPDH was used as a loading control. (D) Relative expression levels of CD133 compared with GAPDH in SP and MP cells, from the western blot analysis. Data are presented as the mean ± standard error of the mean. ** P<0.01, vs. SP cells. FACS, fluorescence-activated cell sorting; SP, side population; MP, main population; CD, cluster of differentiation.

Article Snippet: The cells were incubated with rabbit anti-CD133 fluorescein isothiocyanate (FITC)-conjugated antibody (dilution, 1:500; cat. no. orb15325; Biorbyt, Cambridge, UK) for 30 min.

Techniques: Expressing, Immunofluorescence, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Control, Fluorescence, FACS

CD133 + SP cells show high levels of differentiation. (A) In vitro proliferation assay revealed that the proliferation rate of the CD133 + SP cells were significantly higher, compared with the MP cells. (B) Morphology of the CD133 + SP cells changed rapidly on day 5 and later developed filaments, which resembled fibroblast. Magnification, ×100. Data are presented as the mean ± standard error of the mean. * P<0.05 and ** P<0.01, between SP and MP cells. CD. cluster of differentiation; SP, side population; MP, main population; OD, optical density.

Journal: Molecular Medicine Reports

Article Title: Production of interleukin-4 in CD133 + cervical cancer stem cells promotes resistance to apoptosis and initiates tumor growth

doi: 10.3892/mmr.2016.5195

Figure Lengend Snippet: CD133 + SP cells show high levels of differentiation. (A) In vitro proliferation assay revealed that the proliferation rate of the CD133 + SP cells were significantly higher, compared with the MP cells. (B) Morphology of the CD133 + SP cells changed rapidly on day 5 and later developed filaments, which resembled fibroblast. Magnification, ×100. Data are presented as the mean ± standard error of the mean. * P<0.05 and ** P<0.01, between SP and MP cells. CD. cluster of differentiation; SP, side population; MP, main population; OD, optical density.

Article Snippet: The cells were incubated with rabbit anti-CD133 fluorescein isothiocyanate (FITC)-conjugated antibody (dilution, 1:500; cat. no. orb15325; Biorbyt, Cambridge, UK) for 30 min.

Techniques: In Vitro, Proliferation Assay

CD133 + SP cells exhibit high self-renewal capacity. (A) A clone formation efficiency assay revealed that the total number of tumor spheres generated by the CD133 + SP cells were significantly higher, compared with the number generated by the MP cells. (B) Quantification of the results of reverse transcription-quantitative polymerase chain reaction analysis showed that the relative mRNA expression levels of Oct-4, EpCAM, Sox-2, Bmi-1 and Nestin were significantly upregulated in the CD133 + SP cells, compared with the MP cells. (C) Fluorescence microscopy revealed that the CD133 + SP cells exhibited more positive CD44 fluorescence and EpCAM stem cell proteins, whereas this fluorescence was not enriched in the MP cells. Magnification, ×400. Data are presented as the mean ± standard error of the mean. ** P<0.01, vs. SP. Oct-4, octamer-binding transcription factor-4; EpCam, epithelial cell adhesion molecule; Sox-1, (sex determining region Y)-box 2; Bmi-1, B-cell-specific Moloney murine leukemia virus insertion site-1; CD. cluster of differentiation; SP, side population; MP, main population.

Journal: Molecular Medicine Reports

Article Title: Production of interleukin-4 in CD133 + cervical cancer stem cells promotes resistance to apoptosis and initiates tumor growth

doi: 10.3892/mmr.2016.5195

Figure Lengend Snippet: CD133 + SP cells exhibit high self-renewal capacity. (A) A clone formation efficiency assay revealed that the total number of tumor spheres generated by the CD133 + SP cells were significantly higher, compared with the number generated by the MP cells. (B) Quantification of the results of reverse transcription-quantitative polymerase chain reaction analysis showed that the relative mRNA expression levels of Oct-4, EpCAM, Sox-2, Bmi-1 and Nestin were significantly upregulated in the CD133 + SP cells, compared with the MP cells. (C) Fluorescence microscopy revealed that the CD133 + SP cells exhibited more positive CD44 fluorescence and EpCAM stem cell proteins, whereas this fluorescence was not enriched in the MP cells. Magnification, ×400. Data are presented as the mean ± standard error of the mean. ** P<0.01, vs. SP. Oct-4, octamer-binding transcription factor-4; EpCam, epithelial cell adhesion molecule; Sox-1, (sex determining region Y)-box 2; Bmi-1, B-cell-specific Moloney murine leukemia virus insertion site-1; CD. cluster of differentiation; SP, side population; MP, main population.

Article Snippet: The cells were incubated with rabbit anti-CD133 fluorescein isothiocyanate (FITC)-conjugated antibody (dilution, 1:500; cat. no. orb15325; Biorbyt, Cambridge, UK) for 30 min.

Techniques: Generated, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Fluorescence, Microscopy, Binding Assay, Virus

CD133 + SP cells are multidrug and apoptosis resistant. (A) Comparison of cell survival rate between the CD133 + SP and MP cells following treatment with the DNA targeting drugs, 5-FU, oxaliplatin, cisplatin and paclitaxel. The SP cells showed increased resistance to these drugs, and had a higher survival rate following treatment, compared with the MP cells. (B) Number of CD133 + SP cells undergoing apoptosis were significantly lower, compared with the MP cells. (C) Quantification of results from reverse transcription-quantitative polymerase chain reaction analysis, showing that the relative mRNA expression levels of the ABC transporter gene, ABCG2, and the anti-apoptotic gene, Bcl-2, were significantly upregulated in the CD133 + SP cells. Data are presented as the mean ± standard error of the mean. ** P<0.01, between SP and MP cells. CD, cluster of differentiation; SP, side population; MP, main population; 5-FU, 5-fluorouracil; ABC, ATP-binding cassette.

Journal: Molecular Medicine Reports

Article Title: Production of interleukin-4 in CD133 + cervical cancer stem cells promotes resistance to apoptosis and initiates tumor growth

doi: 10.3892/mmr.2016.5195

Figure Lengend Snippet: CD133 + SP cells are multidrug and apoptosis resistant. (A) Comparison of cell survival rate between the CD133 + SP and MP cells following treatment with the DNA targeting drugs, 5-FU, oxaliplatin, cisplatin and paclitaxel. The SP cells showed increased resistance to these drugs, and had a higher survival rate following treatment, compared with the MP cells. (B) Number of CD133 + SP cells undergoing apoptosis were significantly lower, compared with the MP cells. (C) Quantification of results from reverse transcription-quantitative polymerase chain reaction analysis, showing that the relative mRNA expression levels of the ABC transporter gene, ABCG2, and the anti-apoptotic gene, Bcl-2, were significantly upregulated in the CD133 + SP cells. Data are presented as the mean ± standard error of the mean. ** P<0.01, between SP and MP cells. CD, cluster of differentiation; SP, side population; MP, main population; 5-FU, 5-fluorouracil; ABC, ATP-binding cassette.

Article Snippet: The cells were incubated with rabbit anti-CD133 fluorescein isothiocyanate (FITC)-conjugated antibody (dilution, 1:500; cat. no. orb15325; Biorbyt, Cambridge, UK) for 30 min.

Techniques: Comparison, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Binding Assay

Secretion of IL-4 in CD133 + SP cells. (A) Western blot analysis showed elevated secretion of IL-4 in CD133 + SP cells, compared with MP cells. (B) Relative expression levels of IL-4 compared with GAPDH in SP and MP cells, from the western blot analysis. (C) Cell viability of the CD133 + SP cells was significantly reduced following pretreatment with anti-IL-4 for 24 h. (D) Tumor spheres generated by CD133 + SP cells became more sensitive to apoptosis following pretreatment with anti-IL-4 for 24 h. Magnification, ×25. Data are presented as the mean ± standard error of the mean. * P<0.05 and ** P<0.01, between SP and MP cells. IL-4, interleukin-4; CD, cluster of differentiation; SP, side population; MP, main population; 5-FU, 5-fluorouracil.

Journal: Molecular Medicine Reports

Article Title: Production of interleukin-4 in CD133 + cervical cancer stem cells promotes resistance to apoptosis and initiates tumor growth

doi: 10.3892/mmr.2016.5195

Figure Lengend Snippet: Secretion of IL-4 in CD133 + SP cells. (A) Western blot analysis showed elevated secretion of IL-4 in CD133 + SP cells, compared with MP cells. (B) Relative expression levels of IL-4 compared with GAPDH in SP and MP cells, from the western blot analysis. (C) Cell viability of the CD133 + SP cells was significantly reduced following pretreatment with anti-IL-4 for 24 h. (D) Tumor spheres generated by CD133 + SP cells became more sensitive to apoptosis following pretreatment with anti-IL-4 for 24 h. Magnification, ×25. Data are presented as the mean ± standard error of the mean. * P<0.05 and ** P<0.01, between SP and MP cells. IL-4, interleukin-4; CD, cluster of differentiation; SP, side population; MP, main population; 5-FU, 5-fluorouracil.

Article Snippet: The cells were incubated with rabbit anti-CD133 fluorescein isothiocyanate (FITC)-conjugated antibody (dilution, 1:500; cat. no. orb15325; Biorbyt, Cambridge, UK) for 30 min.

Techniques: Western Blot, Expressing, Generated

CD133 + SP cells are highly tumorigenic and invasive. (A) CD133 + SP cells initiate tumor growth in NON/SCID mice significantly faster, compared with MP cells. (B) Immunohistochemistry showed that SP cell-derived tumor tissues were more positive to the CD133 stem cell protein. Magnification, ×250. (C) CD133 + SP cell invasiveness was measured using a Matrigel assay. The number of CD133 + SP cells, which invaded across the membrane were significantly higher, compared with the number of MP cells. Data are presented as the mean ± standard error of the mean. ** P<0.01, vs. SP. CD, cluster of differentiation; SP, side population; MP, main population.

Journal: Molecular Medicine Reports

Article Title: Production of interleukin-4 in CD133 + cervical cancer stem cells promotes resistance to apoptosis and initiates tumor growth

doi: 10.3892/mmr.2016.5195

Figure Lengend Snippet: CD133 + SP cells are highly tumorigenic and invasive. (A) CD133 + SP cells initiate tumor growth in NON/SCID mice significantly faster, compared with MP cells. (B) Immunohistochemistry showed that SP cell-derived tumor tissues were more positive to the CD133 stem cell protein. Magnification, ×250. (C) CD133 + SP cell invasiveness was measured using a Matrigel assay. The number of CD133 + SP cells, which invaded across the membrane were significantly higher, compared with the number of MP cells. Data are presented as the mean ± standard error of the mean. ** P<0.01, vs. SP. CD, cluster of differentiation; SP, side population; MP, main population.

Article Snippet: The cells were incubated with rabbit anti-CD133 fluorescein isothiocyanate (FITC)-conjugated antibody (dilution, 1:500; cat. no. orb15325; Biorbyt, Cambridge, UK) for 30 min.

Techniques: Immunohistochemistry, Derivative Assay, Matrigel Assay, Membrane

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